Signalling

Part:BBa_K2057007:Design

Designed by: Jos� Eleazar Barboza Corona   Group: iGEM16_Guanajuato_Mx   (2016-10-17)


Signal peptide of ChiA74


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 11


Design Notes

No consideration


Source

Sequence comes from the endochitinase gene chiA74 that is located on a chromosomal sequence of Bacillus thuringiensis

References

Barboza Corona JE, Nieto-Mazzocco E, Velázquez-Robledo R, Salcedo-Hernandez R, Bautista M, Jiménez B, Ibarra JE (2003) Cloning, sequencing and expression of the chitinase chiA74 from Bacillus thuringiensis. Appl Environ Microbiol. 2003 Feb;69(2):1023-1029.